Composite

Part:BBa_K1321336:Design

Designed by: Laura de Arroyo Garcia   Group: iGEM14_Imperial   (2014-10-08)


AraC-pBAD-AcsAB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3049
    Illegal BglII site found at 3763
    Illegal BglII site found at 5686
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 2059
    Illegal BamHI site found at 5053
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 3035
    Illegal AgeI site found at 3112
    Illegal AgeI site found at 3668
    Illegal AgeI site found at 4469
    Illegal AgeI site found at 4561
    Illegal AgeI site found at 4667
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

Source

The AcsAB coding sequence was extracted from NCBI, codon optimised and edited accordingly for further gene synthesis and cloning into pSB1C3.

References